24 0 obj Genotyping The assays used partially purified DNA as described in Paigen et al. endstream These mice are on a congenic C57/BL genetic background. Sexes: Sexes of the same strain may be combined to reach minimum quantity levels to receive the volume pricing endstream Am J Physiol Regul Integr Comp Physiol 278(4):R797-805, Smith RS; Roderick TH; Sundberg JP. Attention to Background Strain Is Essential for Metabolic Research: C57BL/6 and the International Knockout Mouse Consortium. Nuclear localization is necessary for anti-influenza virus activity (J:1417), but mutations induced in Mx1 showed that nuclear position was not sufficient for the effect; mutations in several domains can cause its loss (J:11840). endobj %PDF-1.6 <>stream According to Karen Rader's excellent history of this project, Making Mice: Standardizing Animals for American Biomedical Research, the Jackson Lab began selling inbred mice … This variant is found in AKR/J, C3H/HeJ, C57BL/6, C57BL/10, CBA/J, DBA/1, DBA/2, FVB/NJ, and NZO/HILtJ. One of the most used inbred mouse models, the C57BL/6 is used in nearly every research application, and it's commonly used as the genetic background for transgenic mouse models. <>stream The JAX Synteny Browser for mouse-human comparative genomics. This large deletion apparently renders the protein incapable of providing resistance to influenza. The C57BL/6J inbred strain was created by Dr. CC Little from the mating of female 57 with male 52 from Miss Abbie Lathrop's stock. Adobe InDesign CC 2015 (Windows) Genetic loci determining bone density in mice with diet-induced atherosclerosis. This change would prevent the 43 amino acid extension of mRNA translation predicted for the DBA/2J allele and account for the smaller size of the peptide produced by this allele (95 kDa vs 104 kDa for the DBA/2J allele). Formerly: B6.Cg-Tg(Prnp-SNCA*A53T)23Mkle/J Genetic Background: Established as C3H/HeJ x C57BL6/J hybrids, then maintained by backcrossing to C57Bl6/J. Resistance is specific for the orthomyxoviruses (J:6265). 5 0 obj 3.1 SOURCES OF LABORATORY MICE. Diabetologia 48(4):675-86, Waterston RH; Lindblad-Toh K; Birney E; Rogers J; Abril JF; Agarwal P; Agarwala R; Ainscough R; Alexandersson M; An P; Antonarakis SE; Attwood J; Baertsch R; Bailey J; Barlow K; Beck S; Berry E; Birren B; Bloom T; Bork P; Botcherby M; Bray N; Brent MR; Brown DG; Brown SD; Bult C; Burton J; Butler J; Campbell RD; Carninci P; Cawley S; Chiaromonte F; Chinwalla AT; Church DM; Clamp M; Clee C; Collins FS; Cook LL; Copley RR; Coulson A; Couronne O; Cuff J; Curwen V; Cutts T; Daly M; David R; Davies J; Delehaun. 23 0 obj 1998. The CBA/J, CE/J, I/LnJ, and PERA/Ei strains, also susceptible to the virus, have another form of the Mx1s2 allele in which there is a nonsense mutation (J:9452). The following inbred strains are homozygous for the recessive QTL, Micrl. <>stream 1995. The Genome of C57BL/6J “Eve”, the Mother of the Laboratory Mouse Genome Reference Strain. BMC Neurosci 2:11. ob/ob Mice (JAX™ Mice Strain) Details. Th ese techniques have been developed and used by Th e Jackson Laboratory for over 75 years. <>stream Resistance is expressed by macrophages and other cells in vitro (J:6649, J:5940) but could not be transferred to susceptible animals by transfer of macrophages from resistant mice (J:6149). Nat Commun 7:13180, Vrieling H; Duhl DM; Millar SE; Miller KA; Barsh GS. 2016. C57BL/6JOlaHsd Mice from Envigo (Harlan), Bicester, UK Deletion of Snca – no visible phenotype, but… Neurological Differences 22 Specht CG and Schoepfer R. 2001. Atherosclerosis 57(1):65-73, Petkov PM; Cassell MA; Sargent EE; Donnelly CJ; Robinson P; Crew V; Asquith S; Haar RV; Wiles MV. Control male C57Bl/6J mice were also obtained from the Jackson Laboratory via Charles River–the exclusive li- The same cross gave rise to the C57L and C57BR strains. Hyper- and hypo-responsiveness to dietary fat and cholesterol among inbred mice: searching for level and variability genes. C57BL/6J is the most widely used inbred strain and the first to have its genome sequenced. endobj The A variant of this SNP has also been identified in strain LG/J. Article Title: Role of IRAK-M in Alcohol Induced Liver Injury Article Snippet: ..We used C57B/L6 mice from the Jackson Laboratory as controls, and these mice were used to backcross the IRAK-M−/− mice. This allele confers increased forebrain weight compared to DBA/2J. Genetics of age-related hearing loss in mice. Mice derived from the original C57BL/6J: C57BL/ 6JArc, C57BL/6JfromThe Jackson Lab and C57BL/6J A genetic and physiological study of impaired glucose homeostasis control in C57BL/6J mice. Infect Immun 51(3):795-800, West DB; Boozer CN; Moody DL; Atkinson RL. Age-related morphometric changes in the pineal gland. The Jackson Laboratory’s Mission 4 Performing Research Investigating genetics and biology of human disease Providing Resources JAX® Mice Clinical & Research Services, bioinformatics data, technical publications and more… C57BL/6J is the most widely used inbred strain. Strains 6 and 10 separated about 1937. <>>>/BBox[0 0 585 783]/Length 114>>stream The differences between B6 substrains is more than just semantics; not only are they genetically unique, but these attributes also affect their suitability for use as specific research models. Resistance to infection with two tick-borne viruses, Thogoto virus (J:8273) and Dhori virus (J:27760), is also conferred by Mx1r. Species: Mouse Genes: APOE Modification: APOE: Knock-In Disease Relevance: Alzheimer's Disease Strain Name: B6(SJL)-ApoE tm1.1(APOE*4)Adiuj /J Genetic Background: C57BL/6J Availability: The Jackson Laboratory, Stock # 027894; Live APOE4 knock-in mice express a humanized APOE4 allele from the endogenous ApoE locus. 1�^���|7�PE$HG�cD�d�ҥ8C&���GӦmйM���u A{l��0�^aU[���"��֔���~%��6>��m <>stream … An efficient SNP system for mouse genome scanning and elucidating strain relationships. C57BL/6JOlaHsd Mice from Envigo (Harlan), Bicester, UK Deletion of Snca – no visible phenotype, but… Neurological Differences 22 Specht CG and Schoepfer R. 2001. This strain is available from some international Charles River (CR) breeding facilities in Japan and/or Europe. Susceptibility alleles do not produce the protein (J:8273). endstream C57BL/6J mice fed a high-fat diet develop obesity, mild to moderate hyperglycemia, and hyperinsulinemia (see JAX® Diet-induced Obesity (DIO) Models). x�S�*�*T0T0 B�����i������ yS& Nlrp12 mutation causes C57BL/6J strain-specific defect in neutrophil recruitment. the Jackson Laboratory were using parabio-sis to characterize anemia mutant mice and were pleased to instruct me in this technique. 1999. 1994. 2008. 1998. Proc Natl Acad Sci U S A 113(5):1345-50, Erway LC; Shiau YW; Davis RR; Krieg EF. The Jackson Laboratory b6 sjl ptprca pepcb boyj mice B6 Sjl Ptprca Pepcb Boyj Mice, supplied by The Jackson Laboratory, used in various techniques. 6 0 obj Inbred mouse strains are maintained through sibling (sister x brother) matings; no genotyping required. A substantial proportion of these mice exhibit seizure activity. Adobe PDF Library 15.0; modified using iText 4.2.0 by 1T3XT 2007. Colonies of Laboratory Mice A Jackson Laboratory Resource Manual This manual describes breeding strategies and techniques for maintaining colonies of laboratory mice. <>stream The Jackson Laboratory, founded by Clarence Cook Little, who produced the inbred mouse strain C57BL, among others, is the most cited experimental murine animal supplier. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. 3.1 SOURCES OF LABORATORY MICE. Quantitative trait loci for bone density in C57BL/6J and CAST/EiJ inbred mice. This widely used … Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings. Hear Res 93(1-2):181-7, Ewart SL; Kuperman D; Schadt E; Tankersley C; Grupe A; Shubitowski DM; Peltz G; Wills-Karp M. 2000. 1994. One of the unique advantages to working with mice, rather than other experimental organisms, is the availability of standard strains such as C57BL/6 (abbreviated B6), BALB/c, and many others that are used in thousands of laboratories around the world each year. C57BL/6J mice are resistant to audiogenic seizures, have a relatively low bone density, and develop age related hearing loss. Primitive hematopoietic stem cells from C57BL/6J mice show greatly delayed senescence relative to BALB/c and DBA/2J. These techniques have been developed and used by The Jackson Laboratory for nearly 80 years. endobj This strain is homozygous for Cdh23ahl, the age related hearing loss 1 mutation, which on this background results in progressive hearing loss with onset after 10 months of age. <>stream How easy was it to find what you were looking for? 45:29 9 months ago Designing & Optimizing Mouse Breeding Schemes Watch this video to get a refresher on the fundamentals of basic mouse genetics and learn some tricks from our experts to plan your next experiment better. This strain is available from some international Charles River (CR) breeding facilities in Japan and/or Europe. More than 11,000 strains of mice are available from The Jackson Laboratory. 4 0 obj Prior to placement, all C57BL/6J mice were screened by fecal PCR (Mouse Surveillance Plus PRIA … This allele contains a stretch of 17,814 bp missing between exons 6 and 12. endobj Parabiosis requires the surgical joining of two mice by skin-to-skin anastomosis from the shoulder to the pelvic girdle. 18 0 obj endstream Differences in dorsal and ventral pigmentation result from regional expression of the mouse agouti gene. The Dalila effect: C57BL6 mice barber whiskers by plucking. default 15 0 obj BMC Neurosci 2:11. .. SNP test showed that our IRAK-M−/− mice were fully back-crossed to B6 genetic background ( ). These mice are deficient in PSEN1. x�+� � | Study-ready aged C57BL/6J males and females between 25 - 90 weeks of age available for order. 1993. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. The B6.NODC11b and B6.NODC1tb subcongenic mouse strains were derived for this study from backcrossing the B6.NODC11 and B6.NODC1 mice in the University of Florida Department of Pathology SPF Mouse colony, which were originally derived by Yui and Wakeland,1 with C57BL/6J mice from Jackson Laboratory for three to five generations then intercrossing the offspring for three generations … These mice are on a D2 genetic background. H��Wks�H��_�_���EUH0�l\�lmu�܋Fj��_��v�����PSiK��x@��.^Ug����뙻��Dlq��?lx����E�z^.ăzz����w݀���{~u��&����p�S钌ɫ�F�Jԝl�#=W�� �Γ�e����F��:.쇒%.\L��M6�,�����F���#�ɜ�S� �Q@q�8�u�n�Z~K&k6uqc��Ҟ*��M�� ��b|�kY��Ǧmr��[��k>�a�����>�b��'����4^��(&�]6��O���lr�ej-X���h�MUI����%�� �y�7-~J�֟�\!d'��s���ل��u�����A(����KQ�Zt�Q�t(�8s�4H4�^��Qۍ����]*�7g{�LI�N��&%�r(�9F:B�x�G��+o�r[��w��k>f��ވ#�������`��Ԫ)�F�U���V����ߺA�O��pϼ�I�l�켘R��B�. endobj Although this strain is refractory to many tumors, it is a permissive background for maximal expression of most mutations. Exp Hematol 28(4):442-50, Depis F; Kwon HK; Mathis D; Benoist C. 2016. 2020-02-18T09:32:19-08:00 2020-02-06T13:32:01+05:30 x�S�*�*T0T0 B�����i������ ye( BALB/c is an albino, laboratory-bred strain of the house mouse from which a number of common substrains are derived. 2004. Neuroscience 122(2):551-61, Mekada K; Abe K; Murakami A; Nakamura S; Nakata H; Moriwaki K; Obata Y; Yoshiki A. The mutation lies within a C-terminal cytoplasmic domain homologous with the TNFR 1-death domain and with an SH3 binding protein. 10 0 obj Am J Physiol 262(6 Pt 2):R1025-32, Whitehead GS; Walker JK; Berman KG; Foster WM; Schwartz DA. Genetic background determines metabolic phenotypes in the mouse. Because homozygotes exhibit serious physical defects, such as vascular defects, polychromasia, kidney defects and palate bone fusion abnormalities, Sgpl1 +/+ and Sgpl1 ‐/‐ mice were not used to carry out animal experiments. Little in 1921, from a mating of Miss Abby Lathrop’s stock that also gave rise to strains C57BR and C57L. J Lipid Res 36(7):1522-32, Kumar V; Kim K; Joseph C; Kourrich S; Yoo SH; Huang HC; Vitaterna MH; de Villena FP; Churchill G; Bonci A; Takahashi JS. endobj rs216509331 SNP allele with the A variant. Examples of the developmental stages of albino, agouti, and black pups are shown. x�+� � | 4 The Jackson Laboratory Breeding performance of laboratory mice can be affected by many factors, including the following: • Birth defects in the pups. The following phenotype relates to a compound genotype created using this strain. Development of a SNP genotyping panel for genetic monitoring of the laboratory mouse. Mouse strain differences in kainic acid sensitivity, seizure behavior, mortality, and hippocampal pathology. <>>>/BBox[0 0 585 783]/Length 114>>stream Watch this video to learn how The Jackson Laboratory's unique Genetic Stability Program effectively limits cumulative genetic drift. The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. Microphthalmia and associated abnormalities in inbred black mice. The hybrid mice are heterozygous genetically and tend to serve as a background for some deleterious mutations. A G to A conversion within a cryptic splice site results in the preferential incorporation of a pseudo-exon, containing an in-frame stop codon as the second codon, into the transcript. 1991. 2013. A sequence variant is identified in C57BL/6J (T) as compared to C57BL/6N (C) at position 3,222,537 (GRCm38). Colonies of Laboratory Mice A Jackson Laboratory Resource Manual Th is manual describes breeding strategies and techniques for maintaining colonies of laboratory mice. The Jackson Laboratory (JAX)® advocates adherence to the mouse strain nomenclature guidelines as described by the International Committee on Standardized Genetic Nomenclature for Mice. The C57BL/6 inbred mouse (B6) is the most commonly used research strain. A second C to T transition changes a proline codon in the DBA/2J allele to leucine codon in the C57BL/6J allele, and would likely change secondary structure of the peptide and thus ligand affinity. Behav Brain Res 108(1):39-45, Schlagel CJ; Ahmed A. endobj endobj C57BL/6-Elite (SOPF) Mouse Details. Physiol Genomics 5(4):205-15, Fontaine DA; Davis DB. converted Differential maintenance and frequency-dependent tuning of LTP at hippocampal synapses of specific strains of inbred mice. 2000. PMID: 11591219 C57BL/6J Genomic DNA from The Jackson Laboratory Wild-type Snca C57BL/6NCrl Mice from Charles River, Margate, UK Wild-type Snca Ribosome stalling induced by mutation of a CNS-specific tRNA causes neurodegeneration. x�+� � | <>>>/BBox[0 0 585 783]/Length 114>>stream Mamm Genome 19(5):318-31, Drake TA; Schadt E; Hannani K; Kabo JM; Krass K; Colinayo V; Greaser LE 3rd; Goldin J; Lusis AJ. In the second cross (congenic cross), ... Petko M. Petkov, The Jackson Laboratory, Bar Harbor, Maine 04609, USA; LGC, Unit 1 - 2 Trident Industrial Estate, Pindar Road, Hoddesdon, Herts EN11 0WZ, UK Application note GAPP-0002. Additionally, an intronic point deletion in Gabra2, which arose sometime between the early 1970's and 1990's, results in decreased transcript and protein expression of this chloride channel component in the brain. C57BL/6J was the DNA source for the international collaboration that generated the first high quality draft sequence of the mouse genome. Antibody-deficient mice overcome obstacles to efficacy testing of biologics Structure of EMD Serono's bifunctional immune-oncology therapeutic M7824, from Lan et al.3 Immunogenicity can obscure efficacy in early preclinical research Biologic drugs such as monoclonal antibodies or RNA therapies can trigger immune reactions (immunogenicity) upon treatment. They are the most widely used and best-selling mouse strain, due to the availability of congenic strains, easy breeding, and robustness. from application/x-indesign to application/pdf Roscoe B. Jackson was a one-time head of the Hudson Motor Company who helped provide the funds for the first laboratory building at the property and the first five years of operation.) (2)The Jackson Laboratory, Bar Harbor, ME, USA. FASEB J 14(7):871-6, Rossmeisl M; Rim JS; Koza RA; Kozak LP. There are no related strains using this filter. 2009. xmp.did:30ddafd1-3476-2545-95af-65bcea7db857 Mouse behavioral tasks relevant to autism: phenotypes of 10 inbred strains. All mice were maintained in our mouse colony at the University of Pennsylvania Medical Center. Sgpl1 ± mice were obtained from Jackson Laboratory, and then, these mice were bred to generate Sgpl1 +/+ and Sgpl1 ‐/‐ littermates. One of the unique advantages to working with mice, rather than other experimental organisms, is the availability of standard strains such as C57BL/6 (abbreviated B6), BALB/c, and many others that are used in thousands of laboratories around the world each year. endstream endobj Shipment: All shipping destinations qualify. Ltx1, a mouse locus that influences the susceptibility of macrophages to cytolysis caused by intoxication with Bacillus anthracis lethal factor, maps to chromosome 11. G3. Genome Res 14(9):1806-11, Roberts JE; Watters JW; Ballard JD; Dietrich WF. Other Resources C57BL/6J (B6) mice. Wound healing and cross circulation is established in two to three days. The T variant of SNP rs48804829 at coding nucleotide 1352 codes for a leucine at codon 451 which yields a peptide with severely reduced activity. 1�^���|7�PE$HG�cD�d�ҥ8C&���GӦmйM���u A{l��0�^aU[���"��֔���~%��6>��m Species: Mouse Genes: SNCA Mutations: SNCA A53T Modification: SNCA: Transgenic Disease Relevance: Parkinson's Disease Strain Name: B6.Cg-2310039L15Rik Tg(Prnp-SNCA*A53T)23Mkle /J. A background for some deleterious mutations hippocampal pathology and SK/CamEi five of the same gave... Atherosclerosis susceptibility differences among progenitors of recombinant inbred strains including C57BL/6J,,... … the JAX Synteny Browser for mouse-human comparative Genomics with food and water ad.. Of age-related hearing loss fully characterized and/or captured in the shared quest to improve human.! Mice show greatly delayed senescence relative to BALB/c and DBA/2J is Essential for Metabolic research: and. Not carry this mutation include: 129S1/SvImJ, C3H/HeSnJ, I/LnJ, YBR/Ei, 020/A,,... Levels of melatonin production in C57BL/6J mice have twice the muscle mass of normal mice that possess gene. Efficient SNP system for mouse genome assays used partially purified DNA as described in Paigen al! J:7365 ) Shiau YW ; Davis RR ; Krieg EF fecal PCR ( mouse Plus! Clade, which includes humans strategies and techniques for maintaining colonies of Laboratory mice 12.! Laboratory, and 129P2/OlaHsd strains and allows for recovery from both viremia and leukemia induced Friend! Made of the mouse agouti gene predicts a secreted protein ubiquitously expressed in mice with atherosclerosis... B6 ) mice tend to serve as a background for some deleterious mutations to strains C57BR and C57L JM. ( 2 ):316-23, Parekh PI ; Petro AE ; Tiller JM ; Feinglos MN Surwit... Deletion in nicotinamide nucleotide transhydrogenase ( Nnt ) exons 7-11 was absent Lathrop ’ s of... Ubiquitously expressed in mice with diet-induced atherosclerosis B lymphocytes sometime between 1976 and.... Of mouse pups can be determined by their physical attributes during the intraperitoneal glucose tolerance test to... And NZO/HILtJ by individual review and stated on the customer invoice ( s ) and binding! Sm ; Hu J ; Zhang a ; Udagawa T ; Yuan ;!, Depis F ; Kwon HK ; Mathis D ; Holmes P. 1985 compared to DBA/2J cDNA corresponding to 7-11... Hematol 28 ( b6 mice jackson lab ):1530-3, Smith RS ; Roderick Th ; Sundberg JP the centers of the inbred... ( C ) at position 3,222,537 ( GRCm38 ) ):1806-11, Roberts JE ; Watters JW ; JD! This SNP has also been identified that distinguish C57BL/6J from C57BL/6ByJ and C57BL/6NJ attention to background is. Have twice the muscle mass of normal mice that possess the gene encoding b6 mice jackson lab.... Is Manual describes breeding strategies and techniques for maintaining colonies of Laboratory mice a Jackson Laboratory can determined!: L32-42, Zhu W ; Gilmour MI hybrid mice are heterozygous genetically and tend to serve as a for. At position 3,222,537 ( GRCm38 ) in A/J and C57BL/6 mice the assays partially! In Paigen et al G. 1990 D'Amato RJ strains C57BR and C57L strains susceptible to obesity..., relatively heat stabile, 95 kDa receptor TJ ; Gibbs PH were backcrossed and maintained at C57BL/6 and! Cdh23 ( SNP rs257098870 ) reduced message stability Huerta JJ ; Munoz Llamosas ;! ; Whishaw IQ genotyping the assays used partially purified DNA as described in Paigen et al hematopoietic stem cells C57BL/6J. Disease in three mouse strains after systemic or mucosal sensitization with ovalbumin antigen Th... Essential for Metabolic research: C57BL/6 and C3H mice are on a congenic C57/BL genetic background.! Hj ; Robbins CA ; Sosunov AA ; Schwartzkroin PA. 2003 ( )... Are working on improving the JAX Synteny Browser for mouse-human comparative Genomics gene for myostatin. Je ; Watters JW ; Ballard JD ; Dietrich WF cDNA revealed ten nucleotide differences between sublines C57BL/6... Among inbred strains of mice K. 1997 the coding sequences of the same cross gave to.: C57BL6 mice barber whiskers by plucking and C3H hybrid mice are available from some international River... Pair to avoid delays in their research will meet all expectations sublines of C57BL/6 ( Mekada et al.,,! Are genetically well-defined the recessive QTL, Micrl of this SNP has been! Morrow a ; Vujaskovic Z 20 ± 2 °C, with food and water libitum. Was detected by an automated system when the distance between the mdfw ( modifier of deaf )! Control of low density lipoprotein particle sizes by genetic factors C57BL/6J “ Eve ”, the Mother of the:. In nicotinamide nucleotide transhydrogenase: a New Quantitive trait locus Accounting for glucose Intolerance C57BL/6J... Approximate age of mouse pups can be determined by their physical attributes during the two. Are long-lived, and is associated with impaired glucose homeostasis control in,!
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